Production of tetanus toxin by using media substantially free from meat and blood
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چکیده
منابع مشابه
Glutamine in the production of tetanus toxin.
A strain of Cbstridium tetani under investigation in this laboratory produces high titers of exotoxin when cultivated on a medium containing trypsin-digested protein and high concentrations of iron salts (1). Toxin production fails when acidor alkali-hydrolyzed protein (casein) is substituted for the enzyme digest, and it has been shown (2) that at least two acid-labile substances or groups of ...
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Rapid, simplified method for production and purification of tetanus toxin.
A rapid, simplified method for production and purification of tetanus toxin from bacterial extracts was described. The extracts were prepared by stirring young cells (ca. 45-h culture) of Clostridium tetani in 1 M NaCl-0.1 M sodium citrate, pH 7.5, overnight at 0 to 4 degrees C. The toxin was purified by a combination of (i) ammonium sulfate fractionation (0 to 40% saturation), (ii) ultracentri...
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A modification of the Mueller medium for tetanus toxin production is presented, on which an adapted strain produces high yields of toxin (70 to 90 flocculating units per ml) in the absence of animal protein extracts. The range of iron concentration in the medium was established within which the toxigenic activity of the culture can be made to vary, and the mathematical nature of the variation i...
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In earlier commlnications from this laboratory (Mueller and Miller, 1945, 1947) methods have been described for the preparation of highly potent tetanus toxin, methods which have come into rather general use in large-scale toxoid production for human immunization. The procedure involves the use of a variant and somewhat unstable strain of Clostridium tetani and a culture medium containing a pan...
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ژورنال
عنوان ژورنال: Asian Journal of Pharmaceutical and Clinical Research
سال: 2016
ISSN: 2455-3891,0974-2441
DOI: 10.22159/ajpcr.2016.v9i6.14410